Fibroblast-derived matrices-based human skin equivalent as an in vitro psoriatic model for drug testing

Psoriasis is a chronic skin disease characterized by thickening and disorganization of the skin’s protective barrier. Although current models replicate some aspects disease, development therapeutic strategies have been hindered absence more relevant models. This study aimed to develop characterize an in vitro psoriatic human equivalent (HSE) using keratinocytes HaCat cell line grown on fibroblasts-derived matrices (FDM). The constructed HSEs were treated with cytokines (IL-1α, TNF-α, IL-6, IL-22) allow controlled induction psoriasis-associated features. Histological stainings showed that FDM-HSE composed fully differentiated epidermis fibroblast-populated dermis comparable native rat tail collagen-HSE. Hyperproliferation (CK16 Ki67) inflammatory markers (TNF-α IL-6) expression significantly enhanced cytokine-induced FDM- collagen compared non-treated HSE counterparts. characteristics those observed psoriasis punch biopsies. Treatment all-trans retinoic acid (ATRA) has shown suppress these effects, where both ATRA exhibit histological characteristics, hyperproliferation differentiation like control HSEs. Cytokine-induced FDM-HSE, entirely from lines, provides excellent opportunity for research testing new therapeutics.